Analysis of 16S RNA. Isolates were also tested at the University of Missouri by sequential analysis of part of the 16S-rRNA gene. Isolates were grown on CBA at 37°C for 24 hours, and only one representative colony was vaccinated in 100 μl 1× Tris-EDTA (Promega, Madison, WI, USA) for lysate preparation. The standard PCR, with an initial denaturation step of 15 min at 94 °C, was carried out with previously developed primers, including the Primer 8F (5′-AGAGTTT-GATCMGGCTCAG-3′) and the inverse primer 1391R (5′-GACGGGG-TGTGTRCA-3′) (14). The resulting PCR products were cleaned with a commercial kit (Invitrogen, Carlsbad, CA, USA) and submitted to the University of Missouri DNA Core Facility for wild boar sequencing. The sequences were compared to the GenBank database using the publicly available BLAST (www.ncbi.nlm.nih.gov) nucleotide algorithm. The modified clSI method was used for diagnosis, with one species having an identity ≥99%, regardless of differences in similarity-score (20). The diagnostic results of the other test methods were not known to those who performed the 16S-rRNA test. Finally, the NDA notes the confidentiality of the test and its data. Here you define what your testers can talk about and what they should keep to themselves and for how long.
This clause sets out the parties to the agreement and the desired outcome of the agreement. It also contains a definition of all the specific terms used in the agreement. Terms such as „beta test“, „beta feedback“, etc., are explained in detail in order to clarify what they refer to throughout the agreement. Here is an example of Paragon Software: IMPORTANT: This beta testing agreement regulates the use, testing, and evaluation of Park Assist`s [INX product] by the customer through this portal or hosted environment. Park Assist provides the Services and access to any Software only on the terms set forth in this Agreement and provided that the Customer accepts and complies with them. By clicking the „Accept“ button displayed at the end of this Agreement, you accept (a) this Agreement and agree that the Customer is legally bound by its terms; and (b) declare and warrant that: (i) you are of legal age to enter into a binding agreement and (ii) if the customer is a business, government organization or other legal entity, you have the right, power and power to enter into such agreement on behalf of the customer and to bind the customer on its terms. . . .